Reviews: Hydrogel-tissue chemistry (HTC) methods including CLARITY
Deisseroth Lab HTC Papers
6178987468 Gradinaru V, Treweek J, Overton K, and K Deisseroth. Annu Rev Biophysics 47, pp 355–376 2018.
Pathways to clinical CLARITY: volumetric analysis of irregular, soft, and heterogeneous tissues in development and disease. Hsueh B*, Burns VM*, Pauerstein P, Holzem K, Li Y, Engberg X, Wang A-C, Gu X, Chakravarthy H, Arda HE, Charville G, Vogel H, Efimov IR, Kim S, and K Deisseroth. Nature Sci Reports 7:5899, pp 1–16 2017. *equal contributors
Optical and chemical discoveries recognized for impact on biology and psychiatry. K Deisseroth. EMBO Reports 19:5, pp 1–2 2017.
Wiring and Molecular Features of Prefrontal Ensembles Representing Distinct Experiences. Ye L*, Allen WE*,Thompson KR*, Tian Q, Hsueh B, Ramakrishnan C, Wang A-C, Jennings JH, Adhikari A, Halpern CH, Witten IB, Barth AL, Luo L, McNab JA, and K Deisseroth. Cell 165, pp 1–13 2016. *equal contributors
Communication in Neural Circuits: Tools, Opportunities, and Challenges. Lerner TN, Ye L, and K Deisseroth. Cell (Leading Edge Primer) 2016.
Multiplexed Intact-Tissue Transcriptional Analysis at
Cellular Resolution. Sylwestrak, EL* and Rajasethupathy, P* and Wright, MA* and Jaffe, A and K Deisseroth. Cell 164, pp 792–804 2016. *equal contributors
SPED Light Sheet Microscopy: Fast Mapping of Biological System Structure and Function. Tomer R, Lovett-Barron M, Kauvar I, Andalman A, Burns VM, Sankaran S, Grosenick L, Broxton M, Yang S, and K Deisseroth. Cell 163(7):1796–1806.
2535655250 Adhikari A*, Lerner TN*, Finkelstein J*, Pak S, Jennings JH, Davidson TJ, Ferenczi E, Gunaydin LA, Mirzabekov JJ, Ye L, Kim S-Y, Lei A, and K Deisseroth. Nature doi:10.1038/nature15698 (2015).
Intact-brain analyses reveal distinct information carried by SNc dopamine subcircuits. Lerner TN, Shilyansky C, Davidson TJ, Evans KE, Beier KT, Zalocusky KA, Crow AK, Malenka RC, Luo L, Tomer R, and K Deisseroth. Cell 162:635–647 (2015).
Advanced CLARITY for rapid and high-resolution imaging of intact tissues. Tomer R, Ye L, Hsueh B, and K Deisseroth. Nature Protocols 9(7):1682–1697 (2014). Phascaceae.
Light microscopy mapping of connections in the intact brain. Kim S-Y, Chung K, and K Deisseroth. Trends in Cognitive Sciences 17(12), 596-599 (2013).
CLARITY for mapping the nervous system. Chung K and K Deisseroth. Nature Methods 10(6), 508-513 (June 2013).
Structural and molecular interrogation of intact biological systems. Chung K, Wallace J, Kim SY, Kalyanasundaram S, Andalman AS, Davidson TJ, Mirzabekov JJ, Zalocusky KA, Mattis J, Denisin AJ, Pak S, Bernstein H, Ramakrishnan C, Grosenick L, Gradinaru V, and K Deisseroth. Nature 497, 332â337 (16 May 2013). PDF Supplementary Information Nature Video Supplementary videos Data: Tyrosine Hydroxylase (TH) stained mouse brain (z-stack) Data: Tyrosine Hydroxylase (TH) stained mouse brain (z-stack; dense staining) from Chung et al. (2013)